MALDI-HRMS

• Description

The equipment Exploris 120 Mass Spectrometer from Thermo Scientific with a Spectroglyph MALDI source, combines a high-resolution mass analyzer for accurate mass detection, coupled to a matrix-assisted laser desorption/ionization source (MALDI). It allows the acquisition of data in MS and MS/MS modes, to facilitate the molecular identification and quantification of organic compounds in complex matrices. In addition, it supports the acquisition of mass imaging spectrometry (MSI) experiments.

Characteristics:

• MALDI source that allows the ionization of thermolabile molecules.
• High resolution spectrometer (HRMS) with a minimum resolution of 40,000 (at a mass of 500 m/z) and a Orbitrap-type analyzer.
• Tandem MS experiments by coupling the high-resolution mass spectrometer with a nominal MS resolution (such as quadrupole or ion trap)
• m/z range of at least 50-3000.
• Acquisition experiments in Imaging mode Mass Spectrometry (MSI).

• Applications

• Qualitative analysis
• Identification of metabolites and peptides
• Tissue images
• Analysis of small polymers
• Protein characterization
• Untargeted studies of low molecular weight compounds in solid state biological samples (biological tissue samples, histological sections, biopsy samples, etc.)
• Top - down sequencing

This project is co-financed by the European Regional Development Fund of the European Union in the framework of the State Plan for Scientific and Technical Research and Innovation 2017-2020 with an eligible expenditure of 500.000€.